| Name | Gongzhulingmeisu |
| Synonyms | gongzhlingmeisu Gongzhulingmeisu |
| Physical and Chemical Properties | The chemical quality product is amorphous white powder, and the industrial product is light yellow. It is an alkaline water-soluble antibiotic, which is composed of dehydrated actinone, actinone, bacteriomycin, fluoromycin, naramycin B and benzoic acid. Soluble in a variety of organic solvents. It is stable to acid, heat and light. For example, under acidic conditions, the activity of boiling at 100 ℃ for 30min is basically unchanged, but under alkaline conditions, boiling for 10min will destroy the activity. The half-life in soil is 6 ~ 10d. |
| Use | Uses broad-spectrum antibiotics. With the protective effect of fungicides, there is a certain role in the absorption, mainly used for seed Disinfection. When the treatment agent penetrates into the seed coat, seed kernel and embryo of the seed, it can inhibit the chlamydospore germination of cereal head smut and inhibit the mycelial elongation of germinating chlamydospore, even kill chlamydospores. On the surface of the seed carrying wheat light and net smell smut, sorghum scattered smut and smut, Millet smut control effect of more than 95%, at the same time, it also has a certain control effect on the soil infectious sorghum and maize head smut. When treating seeds, Soak 50 times of water solution with 0.25% wettable powder for more than 12h, fully agitate, spray 8kg of chemical solution per of seeds, stir and stir for 4H for sowing. |
the process of biosynthesis of gongzhilingmycin is as follows: sand tube strain & rarr; Inclined strain & rarr; Shake flask seeds & rarr; Seed tank seeds & rarr; Breeding tank seeds & rarr; Fermentation & rarr; Put the fermentation liquid (pH 3~4) in the tank, acidify & rarr; Concentration & rarr; Drying
A new variety of non-absorbent Streptomyces gongzhuling (strepto myces arygroscopicus gougzrueingensis n. Var.).
Formula of inclined culture medium for inclined strain culture:
Yeast powder 0.1%; Glucose 1%; Beef paste 0.1%; Starch 1%; Peptone 0.2%; Agar 2%;pH natural
After inoculation, culture at 28 ℃ for 10~12 days.
Shake flask seed culture shake flask medium formula:
Soybean cake powder 2%; Corn flour 1.5%; Mother sugar 0.4%; Calcium carbonate 0.3%; Glucose 2%;KH2PO4 0.1%; Sodium chloride 0.1%;pH natural
After inoculation, the shaking chamber at 28 ℃ was oscillated for 24h.
Seed tank seed culture medium formula:
The formula of soybean cake powder, yeast powder, KH2PO4, glucose, corn flour and sodium chloride is the same as above, calcium carbonate 0.1%,pH natural
The tank pressure is 0.05MPa, the tank temperature is 28 ℃, the ventilation rate is 1:1, and the mechanical stirring culture is 24 hours.
Breeding tank seed culture medium formula: the medium formula changes the above glucose 2% to maltose 4%, and the others remain unchanged.
Tank pressure, tank temperature, ventilation and culture time are the same as above.
Fermentation medium formula
Soybean cake powder 6%; Maltose 4%; Corn flour 1. 5%; Yeast powder 0.4%; Calcium carbonate 0.1%;KH2PO4 0.1%; Sodium chloride 0.1%; Soak the enemy 0.025%; The pH value is natural
The tank pressure is 0.05MPa, the tank temperature is 28 ℃, the ventilation rate is 1:1 (less in the front and late stages, more in the middle stage), and the fermentation period is about 70h. The pH value of oxalic acid acidification after the tank is 3~4.
Fermentation broth concentration After acidification, the fermentation broth is concentrated by vacuum membrane. The feed liquid temperature is 40~60 ℃, the pressure is 1.33 × 10-4~1.47 × 10-4Pa, and the flow rate is 250 kg/h. Drying, packaging spray drying, hot air temperature about 150 ℃, exhaust air temperature 75 ℃. After drying, the original medicine is crushed, and the titer is adjusted with talcum powder.