preparation | take the dried leaves of C. Bovis 500g to be crushed into coarse powder, add water to extract 2 times, combine the extracts, concentrate under reduced pressure to a density of 1.14(60 ℃), add ethanol to make the alcohol concentration reach 60%, refrigerated at 0~2 ℃ for 24h, filter, filtrate is concentrated under reduced pressure to obtain extract; Dissolve the extract with methanol, silica gel was added to stir sample, and after drying, column chromatography was performed. Gradient elution was carried out by petroleum ether-ethyl acetate system, and the eluent was combined under the reference of thin layer chromatography. The petroleum ether-ethyl acetate (89: 11~87: 13) repeated recrystallization from ethanol was carried out to obtain the compound of phthalide (white crystals, 1.0g). |