pQE-82L - Names and Identifiers
pQE-82L - Physico-chemical Properties
pQE-82L - Introduction
pQE-82L is a plasmid with the following properties:
1. Structure: pQE-82L is a plasmid with multiple restriction enzyme cleavage sites, usually containing a drug resistance gene with a high copy number, which is used for selective culture in bacteria.
2. use: pQE-82L is usually used for plasmid cloning and expression of foreign protein research. This plasmid can be used for DNA insertion by restriction and ligase reactions, for the construction of recombinant plasmids, and by transformation or transfection into appropriate host cells.
3. Preparation method: The preparation of pQE-82L can be achieved by two main methods. One method is by inserting a DNA fragment of interest into a restriction-cut plasmid by restriction and ligase reaction, and then transforming into a host cell. Another approach is to purchase commercially prepared pQE-82L plasmids.
4. Safety information: When using the pQE-82L, you need to pay attention to the following safety matters: First, the working environment should meet the biosafety standards and follow the relevant laboratory regulations. Second, care should be taken to avoid direct contact with plasmids and possible host bacteria during preparation and manipulation. In addition, strictly follow laboratory waste disposal regulations and do not discard plasmids and plasmid-containing bacteria at will. Finally, after the laboratory operation is completed, clean and disinfect the experimental equipment and work area in time to prevent the leakage and spread of plasmids.
Last Update:2024-04-09 21:00:56