| Storage Condition | 2-8 ℃, dark, 12 months |
| Use | This product is for scientific research only and shall not be used for other purposes. |
| Caution | Due to the unstable microstructure of the microfilament dyeing solution during the dyeing process, some fibers cannot be recognized under an optical microscope, so the fibers that can be seen are main |
| Overview | It is generally believed that in non-muscle cells, microfilaments can promote cytoplasmic flow and participate in cell deformation, endocytosis and pinocytosis, etc. The microfilaments distributed in the microvilli can also cause the microvilli to expand and oscillate. In muscle cells, they are involved in the formation of fine myofilaments, which together with the thick myofilaments, which are mainly composed of myosin, realize the contractile function of muscle cells. microfilament dyeing solution (R250 method) in the dyeing process due to the instability of the microstructure, some fibers can not be identified under the optical microscope, the fibers that can be seen are therefore mainly stress fibers composed of microfilaments. Microfilament dyeing solution (R250 method) mainly shows the stress fiber composed of microfilaments, due to the attachment of cells to the cell matrix and maintaining the shape of flat spread, the fibers are particularly well developed in adherent cells cultured in vitro. |
| Procedure | 1. Sample: dilute PBS buffer(* 10) to 1 * with deionized water *. Approximately 1cm of onion bulb endothelium was gently torn and incubated to allow it to sink. 2. Extraction: PBSbuffer was discarded, TMbuffer was added, and the cap of the weighing bottle was closed and treated at 37 °c. 3. Rinse: discard tmbus, dilute MBus (3 ×) to 1 × with deionized water, and wash with 1 × MBUs. 4. Fixation: The cells were fixed by adding Microfilamentfluid to dry slightly. 5. Rinse: discard Microfilamentfluid and wash with 1 ×mbuffer. 6. Staining: PBSbuffer was discarded, the cover glass was placed on the water-absorbing filter paper to remove the edge moisture, and the r250staringsolution was added for staining. 7. Deionized water was used to rinse the staining solution, and the specimen was spread on glass slide. |
| note | When dyeing animal cell microfilament microfilament staining solution (R250 method), add reagent to the weighing flask slowly, avoid dropping directly onto the slide or sample; Wash the cells gently to avoid cell shedding. |