Name | CHLOROPHYLL |
Synonyms | Chromule c.i.1956 chlorofyl Chlorophyl deodophyll CHLOROPHYLL chlorofolin chlorophylls |
CAS | 1406-65-1 |
EINECS | 215-800-7 |
Molecular Formula | C54H69MgN4O5 |
Molar Mass | 878.454 |
Storage Condition | 2-8 ℃, dark |
MDL | MFCD00071051 |
Physical and Chemical Properties | Chemical properties chlorophyll a:Magnesium complex salt of1,3,5, acid phytyl ester,R = CH3, waxy blue black fine crystal. Often sword-shaped or leaf-shaped. Soluble in ether, alcohol, acetone, chloroform, carbon disulfide and benzene, insoluble in cold methanol, almost insoluble in petroleum ether. Its alcohol solution is blue-green and shows dark red fluorescence. Melting point 117-120 ℃(150-153 ℃, decomposition). Optical rotation -262 ℃ (chloroform). Maximum absorption value (ether) 660,613,557,531,498,429,409nm. |
Use | The use of chlorophyll is suitable for the preparation of wine, green fruit juice, fruit wine, vegetable juice, chewing gum, toothpaste, filled snacks and high-grade candy coloring or complementary color. |
RTECS | FW6420000 |
Reference Show more | 1. [IF=3.638] Ziang Zhang et al."The effect of chlorophyll on the enzyme-linked immunosorbent assay (ELISA) of procymidone in vegetables and the way to overcome the matrix interference."Journal Of The Science Of Food And Agriculture. 2021 Dec 13 |
Merck | 14,2155 |
introduction
chlorophyll is a natural edible pigment extracted from clover, nettle and dried silkworm sand. The main ingredients are chlorophyll a, chlorophyll B, and sodium copper chlorophyllin.. The product is a dark green viscous substance, easily soluble in water and various organic solvents. Under neutral or alkaline conditions, the color is stable green.
use
Chlorophyll is used to color soap, mineral oil, wax and essential oil. Chlorophyll or chlorophyllin derivatives, such as copper chlorophyllin [11006-34-1], sodium iron chlorophyllin, sodium copper chlorophyllin, used in food, candy, beverages, toothpaste, etc. as colorants and deodorants. Chlorophyllin derivatives can be used as a formula for smelly cosmetics with fungicides Jieermite, halocaban, etc.
production method
Chlorophyll is mostly extracted from plants (such as spinach, etc.) or dried silkworm sand. For example, the following methods can be used to extract chlorophyll from silkworm sand. Organic solvent extraction: clean silkworm sand is taken, mixed with more than 70% industrial ethanol into slurry, filtered out dark green solution, and then dried. Another operation is to mix 45 parts of petroleum ether, 15 parts of methanol and 4 parts of benzene, mix and mix with clean silkworm sand, filter and wash the filtrate 4 times. Add a small amount of sodium sulfate to the organic extract to remove residual water, filter, and recover the solvent from the filtrate to obtain chlorophyll. Physical separation: 0.4mol/L dilute sucrose solution and 0.06mol/L dilute potassium phosphate solution (pH 6-7) are mixed into buffer medium according to 1:1. Add about 1L of buffer medium per 2kg of silkworm sand, mix evenly, filter out green suspension with multi-layer emery cloth, centrifuge in a low-temperature centrifuge for 5-10min, wash the precipitate with water, and centrifuge again to obtain chlorophyll precipitate. Chlorophyll is treated with oxalic acid to obtain magnesium-free pheophyllin, and then magnesium is introduced back into chlorophyll. Remove phytol and magnesium with strong acid to obtain magnesium-removing chlorophyllic acid, phytol can also be reintroduced. Hydrolysis removes phytol and methanol to obtain chlorophyllin.