| description | sperm morphology rapid staining solution (Diff-Quik method) belongs to the WHO recommended sperm morphology staining, the staining principle is that proteins with different isoelectric points in sperm and cells have different charges under the same acidity, and can be selectively combined with corresponding dyes to stain; The amino band of the dissociation of the eosinophilic protein is positively charged, can be combined with negatively charged acid dyes (such as eosinophilic anthracene, eosin) to be stained red; Alkaline protein dissociation of carboxyl with negative charge, can be combined with a positively charged basic dye (basic sulfur azobenzene, methylene blue) to be stained blue; Neutral protein dissociation of positively charged amino group and negatively charged carboxyl group are equal, at the same time, the combination of equal acid dyes and basic dyes is purple, but because the dissociation charge is equal, so the coloring is weak. |
| Application | The staining solution is mainly used for the evaluation of sperm (cell) morphology, and contains fixative solution, which is very suitable for batch impregnation, and the background is clear without sediment, the staining solution is only used in the field of scientific research, not for clinical diagnosis or other purposes. Diff-Quik staining is a rapid staining method improved on the basis of Wright staining, which is one of the commonly used staining methods in Cytological Examination. The staining solution is prepared by the rapid staining method recommended by World Health Organization (WHO), similar to Wright Stain, it is improved by using the principle of Romanowsky Stain technology, and the staining result is also very similar to that of Reye's staining solution, but the time required for diff rapid staining is extremely short, and the staining can be completed within 90s in general. Sperm morphology can be assessed using Diff-Quik staining solution. |