| Storage Condition | 2-8 ℃, dark, 6 months |
| Use | This product is for scientific research only and shall not be used for other purposes. |
| Caution | The principle is that the acidic esterase in the cell hydrolyzes α-naphthol acetate to produce α-naphthol under acidic conditions, and α-naphthol is coupled with hexazo parafuchsin to generate insolub |
| staining principle | acid alpha naphthol acetate esterase staining solution (ANAE method), also known as non-specific esterase staining solution, the principle is that the acid esterase in the cell hydrolyzes α-naphthol acetate to produce α-naphthol under acidic conditions, and α-naphthol is coupled with hexaazo parafine to generate insoluble red precipitate, which is localized in the cytoplasm. Acid A- acetic acid naphthol esterase staining solution (ANAE method) has no specificity for esterase staining, can be used for non-specific esterase staining of blood, bone marrow or cell smear, frozen section, can also be used for sodium fluoride inhibition test. The acid A- naphthol acetate esterase staining solution (ANAE method) is only used in the field of scientific research and is not suitable for clinical diagnosis or other purposes. |
| Operating instructions | 1. Blood, bone marrow or cell smears and frozen sections were fixed with ANAE fixative for 10-15min. 2. Wash with water for 5min. 3. Enter the configured ANAE incubation solution, put it in a wet box, incubate at room temperature in the dark for 1H, and wash with water. 4. Counterstain with methyl green staining solution for 5-15min, wash with water, and check with microscope. |
| note | 1. Blood or bone marrow cell smear should be fresh, thin thickness appropriate, generally within 2 days of staining, otherwise it will affect the activity of the enzyme. 2.ANAE incubation solution is easy to fail or reduce the positive degree, that is, it is ready to use, and is not suitable for long time. 3. The ANAE incubation solution was prone to turbidity after configuration, but did not affect the staining effect. Monocytes were moderately positive and strongly positive, sensitive to ANAE inhibitors. Normal granulocytes were negative. 5. Positive control should be provided for each staining. |