| Storage Condition | RT, dark, 12 months |
| Use | This product is for scientific research only and shall not be used for other purposes. |
| Overview | acidic protein is a kind of protein containing acidic amino acids. Different amino acids with different chemical properties of the side chain group, some with a basic side chain, some with an acidic side chain, the composition of the protein has a different number of basic groups and acidic groups, these groups will make the protein with different Net charge in different pH solution, the whole protein molecule with positive charge, that is, basic protein (isoelectric point to alkaline); The whole protein with negative charge, that is, acidic protein (isoelectric point is acidic). The acidic fast green staining solution uses the combination of acidic protein and the positively charged acid dye fast Green to stain. The most abundant acidic protein in the cells mainly exists in the cytoplasm and nucleolus, therefore, the cytoplasm and nucleolus were mostly stained green after staining. Acidic solid green staining solution is only used in the field of scientific research, should not be used for clinical diagnosis or other purposes. |
| Procedure | 1. Take 1 drop of fresh blood at one end of the fragment, push the fragment, and dry it at room temperature. 2. The smear was immersed in 70% ethanol for 5 minutes and dried at room temperature. 3. The smear was immersed in the acidic molecular differentiation fluid, and water bath at 60 ℃ for 30 minutes. 4. The filter paper was washed with water, and the residual water was absorbed. 5. The smear was immersed in acid green stain solution for 5-15 minutes, 6. The smear was washed with running water and dried at room temperature. 7. Direct microscopic examination or adding 1 drop of neutral gum Dropwise and sealing observation with cover fragment. |
| note | 1, blood smear or bone marrow smear should be uniform in thickness, so as not to affect the effect of staining. 2, blood cell smear staining requires fresh whole blood or EDTA anticoagulant. 3. After incubation with acidic differentiation solution, rinse thoroughly, otherwise it will interfere with the staining of solid green. 4. If the dyeing is too deep, methanol or alcohol can be used for appropriate decolorization, preferably without complication. 5, pH value has a certain effect on dyeing, in the fragment should be clean, no acid and alkali pollution, so as not to affect the dyeing effect. |
| Use | display basic proteins in cells, especially histone |