5(6)-CFDA MedChemExpress (MCE)

Product Name	5(6)-Carboxy-di-O-acetylfluorescein
Synonyms	5(6)-CFDA 5(6)-FAM DA 5(6)-FAM DIACETATE 5(6)-Carboxyfluorescein diacetate 5(6)-Carboxy-di-O-acetylfluorescein 5-(AND-6)-CARBOXYFLUORESCEIN DIACETATE 5(6)-CFDA [5(6)-Carboxyfluorescein diacetate] Synonyms 5(6)-CFDA 5(6)-FAM DA 5(6)-FAM DIACETATE 5(6)-Carboxyfluorescein diacetate 5(6)-Carboxy-di-O-acetylfluorescein 5-(AND-6)-CARBOXYFLUORESCEIN DIACETATE 5(6)-CFDA [5(6)-Carboxyfluorescein diacetate] Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylicacid, 3',6'-bis(acetyloxy)-3-oxo-
CAS	124387-19-5
EINECS
Chemical Formula	C50H32O18
Molecular Weight	920.78
inchi
Package	50 mg;100 mg
Price	Email to quote
Descriptions	5(6)-CFDA 5(6)-CFDA MedChemExpress (MCE) HY-D0722 124387-19-5 5-(6)-Carboxyfluorescein diacetate Descriptions 5(6)-CFDA 5(6)-CFDA MedChemExpress (MCE) HY-D0722 124387-19-5 5-(6)-Carboxyfluorescein diacetate CFDA 99.31% -20°C, protect from light In solvent : -80°C, 6 months -20°C, 1 month (protect from light) Room temperature in continental US may vary elsewhere. 5(6)-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes. Preparation of 5(6)-CFDA working solution 1. Preparation of the stock solution Dissolve 1mg 5(6)-CFDA in 0.21 mL DMSO to obtain 10 mM of 5(6)-CFDA. Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles. 2. Preparation of 5(6)-CFDA working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of 5(6)-CFDA working solution. Note: Please adjust the concentration of 5(6)-CFDA working solution according to the actual situation. Cell staining 1. Cell preparation:For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2. Add 1 mL of 5(6)-CFDA working solution, and then incubate at room temperature for 30 minutes. 3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. 4. Wash twice with PBS, 5 minutes each time. 5. Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer. Precautions 1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles. 2. Please adjust the concentration of 5(6)-CFDA working solution according to the actual situation. 3. This product is for R&D use only, not for drug, household, or other uses. 4. For your safety and health, please wear a lab coat and disposable gloves to operate. 525 488 \| \| \| \| \| \| \| \| \| \| [1]. Yang T, et al. A novel nonradioactive CFDA assay to monitor the cellular immune response in myeloid leukemia. Immunobiology. 2013 Apr 218(4):548-53. [Content Brief]* [2]. Card SD, et al. Assessment of fluorescein-based fluorescent dyes for tracing Neotyphodium endophytes in planta. Mycologia. 2013 Jan-Feb 105(1):221-9. [Content Brief] [3]. Fang X, et al. Bone marrow-derived endothelial progenitor cells are involved in aneurysm repair in rabbits. J Clin Neurosci. 2012 Sep 19(9):1283-6. [Content Brief]
Last Update	2025-04-01 14:45:47

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