Calcein Blue MedChemExpress (MCE)

Product Name	4-methylcoumarin-6-ylmethyliminodiacetic acid
Synonyms	CALCEIN BLUE TIMTEC-BB SBB002950 UMBELLIFERONE COMPLEXONE 4-methylcoumarin-6-ylmethyliminodiacetic acid 4-METHYLUMBELLIFERONE-8-METHYLIMINODIACETIC ACID B-METHYLUMBELLIFERONE METHYLENEIMINODIACETIC ACID Synonyms CALCEIN BLUE TIMTEC-BB SBB002950 UMBELLIFERONE COMPLEXONE 4-methylcoumarin-6-ylmethyliminodiacetic acid 4-METHYLUMBELLIFERONE-8-METHYLIMINODIACETIC ACID B-METHYLUMBELLIFERONE METHYLENEIMINODIACETIC ACID 4-METHYLUMBELLIFERONE METHYLENEIMINODIACETIC ACID 4-METHYLUMBELLIFERONE-6-METHYLENEIMINODIACETIC ACID 4-METHYLUMBELLIFERONE-8-METHYLENEIMINODIACETIC ACID
CAS	54375-47-2
EINECS	259-129-8
Chemical Formula	C15H15NO7
Molecular Weight	321.28
inchi	InChI=1/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)/p-1
Package	10 mM * 1 mL;25 mg
Price	Email to quote
Descriptions	Calcein Blue Calcein Blue MedChemExpress (MCE) HY-101887 54375-47-2 97.03% 4°C, protect from light In solvent : -80°C, 6 months Descriptions Calcein Blue Calcein Blue MedChemExpress (MCE) HY-101887 54375-47-2 97.03% 4°C, protect from light In solvent : -80°C, 6 months -20°C, 1 month (protect from light) Room temperature in continental US may vary elsewhere. Calcein Blue, a membrane-impermeant fluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator. The fluorescence of Calcein Blue (CB) is quenched by the Fe2+ ion in the Calcein Blue-Fe2+ complex. When dopamine is added to a solution of the Calcein Blue-Fe2+ complex, a dopamine-Fe2+ complex is formed as the result of a ligand exchange reaction between Calcein Blue and dopamine which permits the fluorescence from Calcein Blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the Calcein Blue-Fe2+ complex solution, which permits dopamine to be quantitatively determined. The Calcein Blue-Fe2+ complex shows a high selectivity for dopamine among the catecholamines and related compounds examined[1]. The recommended final working concentrations is usually between 1 and 10 μM to minimize potential artifacts. Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processing of the dyes. A stock concentration of 5 mM 4-methylumbelliferone-8- methyliminodiacetic acid, commonly known as calcein blue is prepared in 0.1 M potassium hydroxide, and the pH is neutralized using 0.1 N hydrochloric acid. The calcein blue stock prepared is diluted to 200 μM (working stock) in DPBS and stored for further use. A concentration of 10 μM calcein blue is prepared from the working stock by diluting in DPBS. From this, 200 μL is added to a 96-well plate and the excitation/emission (Ex./Em.) maximum is determined using the micro-plate reader[1]. 441 354 \| \| \| \| \| \| \| \| \| \| [1]. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30 94:36-43. [Content Brief] [2]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar 99(5):2339-49. [Content Brief] [3]. G M Huitink, et al. Methyl calcein blue and other analogues of calcein blue. Talanta. 1974 Nov 21(11):1193-202. [Content Brief]
Last Update	2025-04-01 14:45:47

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