Dihydrorhodamine 123 MedChemExpress (MCE)

Product Name	Dihydrorhodamine 123
Synonyms	DHR DHR123 Dihydrorhodamine Dihydrohodamine 123 DIHYDRORHODAMINE 123 Dihydrorhodamine 123 Dihydrorhodamine123(DHR123) Synonyms DHR DHR123 Dihydrorhodamine Dihydrohodamine 123 DIHYDRORHODAMINE 123 Dihydrorhodamine 123 Dihydrorhodamine123(DHR123) DIHYDRORHODAMINE 123, FOR FLUOR-ESCENCE 2-(3,6-DIAMINO-9H-XANTHEN-9-YL)-BENZOIC ACID, METHYL ESTER
CAS	109244-58-8
EINECS
Chemical Formula	C21H18N2O3
Molecular Weight	346.38
inchi
Package	1 mg;5 mg;10 mg;25 mg;50 mg
Price	Email to quote
Descriptions	Dihydrorhodamine 123 Dihydrorhodamine 123 MedChemExpress (MCE) HY-101894 109244-58-8 DHR 123 99.83% -20°C, protect from light The compound is unstable in solutions, freshly prepared is recommended. Room temperature in continental US Descriptions Dihydrorhodamine 123 Dihydrorhodamine 123 MedChemExpress (MCE) HY-101894 109244-58-8 DHR 123 99.83% -20°C, protect from light The compound is unstable in solutions, freshly prepared is recommended. Room temperature in continental US may vary elsewhere. Dihydrorhodamine 123 (DHR 123) is a fluorescent probe (λex=488 nm, λem=525 nm). In the presence of 10 μM Dihydrorhodamine 123 (DHR 123) the stimulation of the neutrophil NADPH oxidase by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) resultes in an increase in the rate of rhodamine generation. The fluorescent intensity of the cells, in the presence of 10 μM Dihydrorhodamine 123, increases with time following the addition of 50 nM PMA. In the presence of 10 μM Dihydrorhodamine 123, induced HL60 cells show a sustained increase in fluorescence following the addition of 50 nM PMA[1]. The HL60 cells are incubated at 6×106 cells/mL in Krebs-Ringer buffer at 37°C containing 10 μM Dihydrorhodamine 123 (DHR). The generation of O2- is initiated by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) and the progress of the generation of rhodamine 123 is monitored in 50-μL aliquots (3×105 cells) diluted tenfold before analysis. The uninduced HL60 cells are loaded with 5 μM carboxy SNARF-1 AM acetate (SNARF-AM) in the Na+ medium for 10 min at 37°C and washed by centrifugation and resuspension to remove unhydrolysed SNARF ester[1]. 610 515 \| \| \| \| \| \| \| \| \| \| [1]. Lydia M. Henderson et al. Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur.J.Biochem. 217, 973-980.
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Last Update	2025-05-21 16:50:25

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