Fluorescein di(β-D-galactopyranoside) MedChemExpress (MCE)

Product Name	FDG [Fluorescein di-β-D-galactopyranoside]
Synonyms	FDG fluorescein-digalactoside FLUORESCEIN DI-BETA-D-GALACTOSIDE FLUORESCEIN DI-BETA-D-GALACTOPYRANOSIDE FDG [Fluorescein di-β-D-galactopyranoside] Fluorescein di(β-D-galactopyranoside) (FDG) Synonyms FDG fluorescein-digalactoside FLUORESCEIN DI-BETA-D-GALACTOSIDE FLUORESCEIN DI-BETA-D-GALACTOPYRANOSIDE FDG [Fluorescein di-β-D-galactopyranoside] Fluorescein di(β-D-galactopyranoside) (FDG) 3',6'-bis[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]spiro[2-benzofuran-3,9'-xanthene]-1-one
CAS	17817-20-8
EINECS	1312995-182-4
Chemical Formula	C32H32O15
Molecular Weight	656.587
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Package	1 mg;5 mg
Price	Email to quote
Descriptions	Fluorescein di(β-D-galactopyranoside) Fluorescein di(β-D-galactopyranoside) MedChemExpress (MCE) HY-101895 17817-20-8 FDG 99.81% -20°C, protect from light The compound is unstable in solutions, freshly prepared is recommended. Room temperature in continental US Descriptions Fluorescein di(β-D-galactopyranoside) Fluorescein di(β-D-galactopyranoside) MedChemExpress (MCE) HY-101895 17817-20-8 FDG 99.81% -20°C, protect from light The compound is unstable in solutions, freshly prepared is recommended. Room temperature in continental US may vary elsewhere. Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm). The fluorescence produced by Fluorescein di(β-D-galactopyranoside) increases in a time- and dose-dependent manner. The level of fluorescence produced by the double-substrate method is much lower than that by the Fluorescein di(β-D-galactopyranoside) method. Results show that the fluorescein produced by Fluorescein di(β-D-galactopyranoside) in Hs68 cells is proportional to the number of passages[1]. The cells (5×103 cells per well) are cultured in a 96-well plate overnight for attachment, washed, and then fixed in solutions. An aliquot (100 μL) of the reaction buffer (i.e., the staining solution without X-Gal) is added into each well. Then, 10 μL of 2 mM Fluorescein di(β-D-galactopyranoside) is added per well and the plate is incubated in the dark at 37°C for 24 h without CO2 supply. After incubation at 37°C for 24 h, 100 μL of the supernatant is transferred to a 96-well plate for fluorescent measurement in triplicates. The fluorescein fluorescence is measured using a fluorometer with an excitation at 485 nm and an emission at 535 nm[1]. 520 488 \| \| \| \| \| \| \| \| \| \| [1]. Yang NC, et al. A fluorimetric method using fluorescein di-beta-D-galactopyranoside for quantifying the senescence-associated beta-galactosidase activity in human foreskin fibroblast Hs68 cells. Anal Biochem. 2004 Feb 15 325(2):337-43. [Content Brief]
Last Update	2025-04-01 14:45:47

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