Texas RedTexas Red
MedChemExpress (MCE)
HY-101878
60311-02-6
Sulforhodamine 101
SR101
99.72%
-20°C, protect from light *The compound is unstable in solutions, freshly prepared is recommended.
Room temperature in continental US
may vary elsewhere.
Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations.
Texas Red (Sulforhodamine 101) does not label astrocytes in brainstem slices as strong and specific as in the hippocampus or cortex. To minimize excitatory side effects, the concentration of Texas Red has to be kept as low as possible or the labeling procedure can be performed after the actual experiment[1].
In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Texas Red or topical application of 100 μM[1].
For in vivo imaging, Texas Red (Sulforhodamine 101) is applied topically at concentrations of 250 nM to 300 μM or by bolus injection. Additionally, Texas Red injection over the tail vein (10 mg/mL) has been reported to be successful[1].
Acute brain slices are usually incubated in carbonated extracellular solution containing 0.5 to1 μM Texas Red for 20 to 30 min and 34 to 37°C. Following this, excess dye is removed over a period of 10 to 30 min using different protocols that were described earlier[1].
605
586
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[1]. Axel Nimmerjahn, et al. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods. 2004 Oct
1(1):31-7. [Content Brief]
[2]. J Kang, et al. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010 Sep 15
169(4):1601-9. [Content Brief]