4-Methylumbelliferyl phosphate MedChemExpress (MCE)

Product Name	4-methyl-2-oxo-2H-1-benzopyran-7-ylphosphonic acid
Synonyms	4-MUP 4-Methylumbelliferyl-phosphate 4-Methylumbelliferyl phosphate 4-METHYLUMBELLIFERYL PHOSPHATE (4-MUP) LIQUID SUBSTRATE SYSTEM 4-METHYLUMBELLIFERONE PHOSPHATE 4-METHYLUMBELLIFERYL-PHOSPHORIC ACID Synonyms 4-MUP 4-Methylumbelliferyl-phosphate 4-Methylumbelliferyl phosphate 4-METHYLUMBELLIFERYL PHOSPHATE (4-MUP) LIQUID SUBSTRATE SYSTEM 4-METHYLUMBELLIFERONE PHOSPHATE 4-METHYLUMBELLIFERYL-PHOSPHORIC ACID 4-METHYL-7-(PHOSPHONOOXY), 2H-1-BENZOPYRAN-2-ONE 2H-1-Benzopyran-2-one, 4-methyl-7-(phosphonooxy)- 4-methyl-2-oxo-2H-1-benzopyran-7-ylphosphonic acid 4-Methyl-2-oxo-2H-1-benzopyran-7-ylphosphonic acid 4-METHYL-2-OXO-2H-CHROMEN-7-YL DIHYDROGEN PHOSPHATE 4-methyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate 4-METHYLUMBELLIFERYL PHOSPHATE LIQUID SUBSTRATE SYSTEM
CAS	3368-04-5
EINECS	222-137-7
Chemical Formula	C10H9O6P
Molecular Weight	256.15
inchi	InChI=1/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
Package	10 mM * 1 mL;100 mg
Price	Email to quote
Descriptions	4-Methylumbelliferyl phosphate 4-Methylumbelliferyl phosphate MedChemExpress (MCE) HY-D0994 3368-04-5 4-MUP Descriptions 4-Methylumbelliferyl phosphate 4-Methylumbelliferyl phosphate MedChemExpress (MCE) HY-D0994 3368-04-5 4-MUP MUP 99.96% -20°C, protect from light In solvent : -80°C, 6 months -20°C, 1 month (protect from light) Room temperature in continental US may vary elsewhere. 4-Methylumbelliferyl phosphate (4-MUP), an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate is also a nerve agent simulant. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Serum acid phosphatase assay[1]: 1. Preparation a 100 μL solution containing the following agents: 5.0 μL of serum enzyme, 50 μL of 4-Methylumbelliferyl phosphate (5.0 mM), and 10 μL of 1.0 M buffer at pH 6.0. 2. Add the following agents (with the final concentration) 20 mM Sodium Tartrate (HY-128476), 15 mM Sodium Fluoride (HY-B1766), 3000 mM 2-Mercaptoethanol, 4 mM 1, 10-Phenanthroline (HY-W004544), 6 mM 8-Quinolinol (HY-B1005), 6 mM 8-Hydroxy-5quinolinesulfonic acid, 45 mM EDTA (HY-Y0682) and 6 mM EGTA (HY-D0861). 3. Incubate reaction mixtures for 15 min at 37℃ and terminated by the addition of 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer, pH 10.5. 4. Mix samples thoroughly and determine fluorescence by a fluorometer. \| \| \| \| \| \| \| \| \| \| [1]. Chambers JP, et al. Determination of serum acid phosphatase in Gaucher's disease using 4-methylumbelliferyl phosphate. Clin Chim Acta. 1977 Oct 1 80(1):67-77. [Content Brief]* [2]. Watanabe F, et al. The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane. Clin Chim Acta. 1979 Feb 1 91(3):273-6. [Content Brief] [3]. Hao Chen, et al. Autonomic Molecular Transport for Ultrasensitive Surface-Enhanced Infrared Absorption Spectroscopy. ACS Appl. Polym. Mater. 2020, 2, 9, 3929-3935
Last Update	2025-04-01 14:45:47

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