Methyl OrangeMethyl Orange
MedChemExpress (MCE)
HY-118907
547-58-0
99.41%
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Room temperature in continental US
may vary elsewhere.
Methyl Orange is a soluble azo dye commonly used as an acid-base indicator and for staining cells and tissue sections, as well as for dyeing textiles. Methyl Orange appears red at a pH of 3.1 and changes to bright yellow as the pH increases to 4.4. Methyl Orange (500 mg/L) exhibits cytotoxicity and can cause DNA damage.
1. Acid-Base Indicator[2] Methyl Orange exhibits a distinct color change at different pH values: pH pH 3.1 - 4.4: Orange pH > 4.4: Bright Yellow 2. Methyl Orange Vivo Staining Effects and Toxicity Studies[3] 2.1 Preparation of Working Solution Dissolve Methyl Orange in Hoagland nutrient solution to a concentration of 2.0 mmol/L. 2.2 Vivo Staining (1).Select healthy, uniformly sized, well-rooted, and pest-free Spider Plant cuttings and transplant them into Hoagland nutrient solution containing 2.0 mmol/L Methyl Orange at pH 6.5. Change the nutrient solution every 3 days. (2).On days 20, 40, and 60 of treatment, randomly select 10 mature, healthy, pest-free leaves from each plant and measure the leaf color parameters in the middle of the leaf surface using an NC-9801 handheld colorimeter. (3).On day 60 of treatment, measure the biomass and root-to-shoot ratio, where root-to-shoot ratio = (fresh weight of underground part / fresh weight of above-ground part) × 100%. (4).Starting from day 10 of treatment, measure leaf nutrient content every 10 days, including soluble sugars, starch, soluble proteins, total nitrogen, chlorophyll, and carotenoids. 1.3 Data Analysis Perform significance analysis using SPSS 22.0 software to determine the staining effect of Methyl Orange on Spider Plants and its impact on their biological activity.
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