Lucigenin MedChemExpress (MCE)

Product Name	N,N'-dimethyl-9,9'-biacridinium dinitrate
Synonyms	L-6868 LUCIGENIN NSC-151912 BIS(N-METHYLACRIDINIUM) NITRATE BIS(N-METHYLACRIDINIUM) DINITRATE 9,9'-BIS(N-METHYLACRIDINIUM NITRATE) N,N'-dimethyl-9,9'-biacridinium dinitrate Synonyms L-6868 LUCIGENIN NSC-151912 BIS(N-METHYLACRIDINIUM) NITRATE BIS(N-METHYLACRIDINIUM) DINITRATE 9,9'-BIS(N-METHYLACRIDINIUM NITRATE) N,N'-dimethyl-9,9'-biacridinium dinitrate lucigenin or N,N′-dimethyl-9,9′-biacridinium dinitrate 10,10'-Dimethyl-9,9'-biacridinium Dinitrate [for Chemiluminescence Research]
CAS	2315-97-1
EINECS	219-023-4
Chemical Formula	C28H22N4O6
Molecular Weight	510.5
inchi	InChI=1/C28H22N2.2NO3/c1-29-23-15-7-3-11-19(23)27(20-12-4-8-16-24(20)29)28-21-13-5-9-17-25(21)30(2)26-18-10-6-14-22(26)28;22-1(3)4/h3-18H,1-2H3;;/q+2;2-1
Package	10 mM * 1 mL;5 mg;10 mg;50 mg;100 mg;500 mg
Price	Get quote
Descriptions	Lucigenin Lucigenin MedChemExpress (MCE) HY-D0720 2315-97-1 NSC-151912 Descriptions Lucigenin Lucigenin MedChemExpress (MCE) HY-D0720 2315-97-1 NSC-151912 L-6868 99.12% 4°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months -20°C, 1 month (sealed storage, away from moisture and light) Room temperature in continental US may vary elsewhere. Lucigenin is a chemiluminescence probe. Lucigenin can be used to detect the production of endogenous superoxide anion radical (O2-). Lucigenin is extremely sensitive to chloride ions, while it combined with chloride ions, the fluorescence will be quenched. Lucigenin also can be used as a chloride indicator. Ex/Em=455/505 nm. Preparation of Lucigenin working solution1.1 Preparation of the stock solutionDissolve 1 mg of Lucigenin in 0.1919 mL of DMSO to obtain 10 mM of Lucigenin.Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.1.2 Preparation of Lucigenin working solutionDilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of Lucigenin working solution.Note: Please adjust the concentration of Lucigenin working solution according to the actual situation.Cell staining2.1 Cell preparation.For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.2.2 Add 1 mL of Lucigenin working solution, and then incubate at room temperature for 15 minutes.2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.2.4 Wash twice with PBS, 5 minutes each time.2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope. 505 455 \| \| \| \| \| \| \| \| \| \| [1]. Li Y, et al. Validation of lucigenin (bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular systems. J Biol Chem. 1998 Jan 23 273(4):2015-23. [Content Brief]*
Last Update	2025-04-01 14:45:47

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