108964-32-5
fura-2|am
CAS: 108964-32-5
Molecular Formula: C44H47N3O24
108964-32-5 - Names and Identifiers
| Name | fura-2|am |
| Synonyms | Fura2-AM Fura?-AM fura 2-am yellow powder FURA 2-AM SPECIAL PACKAGING FURA-2 PENTAKIS(ACETOXYMETHYL) ESTER acetoxymethyl 2-[5-[bis[2-(acetoxymethoxy)-2-oxo-ethyl]amino]-4-[2-[2-[bis[2-(acetoxymethoxy)-2-oxo-ethyl]amino]-5-methyl-phenoxy]ethoxy]benzofuran-2-yl]oxazole-5-carboxylate |
| CAS | 108964-32-5 |
| InChI | InChI=1/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3 |
108964-32-5 - Physico-chemical Properties
| Molecular Formula | C44H47N3O24 |
| Molar Mass | 1001.85 |
| Density | 1.400±0.06 g/cm3(Predicted) |
| Melting Point | >250℃ |
| Boling Point | 975.9±75.0 °C(Predicted) |
| Flash Point | 544°C |
| Solubility | DMSO: soluble |
| Vapor Presure | 0mmHg at 25°C |
| Appearance | Powder |
| Color | Yellow |
| Maximum wavelength(λmax) | ['370nm'] |
| BRN | 8183748 |
| pKa | 1.89±0.50(Predicted) |
| Storage Condition | −20°C |
| Sensitive | Light Sensitive |
| Refractive Index | 1.566 |
| In vitro study | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid. 1. First, prepare the 1 mM Fura-2 AM stock by adding 50 µL of DMSO to a 50 µg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months. 2. Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1µM Fura-2 AM solution. Vortex the solution vigorously for 1 min. 3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish. 4. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely. 5. Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish. 6. Mount the coverslip on the imaging chamber. |
108964-32-5 - Risk and Safety
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 8-10 |
108964-32-5 - Reference Information
| biological field application | Calcium indicator; monitoring membrane potential; treating ischemia |
| biological activity | Fura-2 AM is a high affinity, intracellular, ultraviolet light excitation and UV ratio fluorescent Ca2 indicator. |
Last Update：2024-04-09 21:01:54
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