中文名 | 派洛宁Y |
英文名 | Pyronin Y |
别名 | 派洛宁G 派洛宁Y 吡罗红Y 派洛宁YPYRONI 派洛宁Y92-32-0 派洛宁Y, 高纯生物染料 派洛宁Y(IND GRADE) 3,6-双(二甲基氨基)吨氯化物 PYRONING(Y) 吡啰红G 派洛宁G (Y) |
英文别名 | Pyronine Pyronin G Pyronin Y Pyronine Y C.I. 45005 PYRONINE G Pyronin y(G) Pyronin Y, CI 45005 Pyronin G (Pyronin Y) Pyronin G, PyroninJ Pyronin Y (CI x 45005) pyronin Y molecular biology Pyronin G, PyroninJ, PyroninY Pyronin G, C.I. 45005, for microscopy Pyronin Y,Pyronin G, PyroninJ, PyroninY Pyronin Y,pure,high purity biological stain N-[6-(dimethylamino)-3H-xanthen-3-ylidene]-N-methylmethanaminium chloride |
CAS | 92-32-0 |
EINECS | 202-147-8 |
化学式 | C17H19ClN2O |
分子量 | 302.8 |
InChI | InChI=1/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1 |
InChIKey | INCIMLINXXICKS-UHFFFAOYSA-M |
熔点 | 250-260°C(lit.) |
水溶性 | Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL). |
溶解度 | 溶于水; 微溶于乙醇、乙二醇、甲基溶纤剂 |
存储条件 | room temp |
稳定性 | 稳定。与强氧化剂不相容。 |
外观 | 粉末/固体 |
颜色 | Pink |
最大波长(λmax) | ['548 nm'] |
Merck | 13,8097 |
BRN | 3795789 |
MDL号 | MFCD00011725 |
体外研究 | Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin. A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting. In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells. Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent. Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA). |
危险品标志 | Xn - 有害物品 Xi - 刺激性物品 |
风险术语 | R68 - 可能有不可逆后果的危险。 R36/37/38 - 刺激眼睛、呼吸系统和皮肤。 |
安全术语 | S22 - 切勿吸入粉尘。 S24/25 - 避免与皮肤和眼睛接触。 S36 - 穿戴适当的防护服。 S26 - 不慎与眼睛接触后,请立即用大量清水冲洗并征求医生意见。 |
WGK Germany | 3 |
RTECS | BQ1450000 |
TSCA | Yes |
海关编号 | 32041300 |
参考资料 展开查看 | 1. 杨成焕, 杨姚, 李阔,等. 红芸豆植物凝集素终止小鼠中晚期妊娠对胎盘组织的影响研究[J]. 中国计划生育学杂志, 2015, 23(8). |
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