酚试剂
福林酚(Folin-Phenol)
CAS:
化学式:
| 中文名 | 福林酚 |
| 英文名 | Folin-Phenol |
| 别名 | 福淋酚 福林酚 酚试剂 |
| 英文别名 | Phennol Folin-Phenol Foline-Phenol Folin-Ciocalteu'S phenol reagent |
| 存储条件 | Room Temprature |
| 外观 | 液体 |
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酚试剂 - 简介
福林酚(Folin-Phenol)是一种用于测定蛋白质含量的化学试剂。它通常用于蛋白质浓度的分析和测定，通过与蛋白质中的芳香族氨基酸（如酪氨酸和苯丙氨酸）发生氧化反应，生成显色产物后测定其吸光度，从而确定蛋白质含量。这种方法也常用于测定生物样品中的总蛋白含量，如血清、细胞提取物等。福林酚法是一种经典的蛋白质定量方法，被广泛应用于生化实验和研究中。
性质：福林酚是一种深蓝色液体，具有较高的氧化性。它在与蛋白质中的芳香族氨基酸发生氧化反应后会产生浓郁的蓝紫色，并且对光线敏感。
用途：福林酚主要用于测定蛋白质含量，可用于各种生化实验中。该试剂能够通过与蛋白质中的芳香族氨基酸的氧化反应，快速、准确地测定蛋白质的含量。它还可用于检测生物样本中的总蛋白含量，如血清、细胞提取物等。
制法：福林酚是由福林-西格韦氏试剂（Folin-Ciocalteu reagent）与酚溶液混合而成的，通常由磷酸钒酸铵和磷酸硼酸钠等原料制备而成。
安全性：福林酚属于强氧化剂，具有刺激性和腐蚀性。在使用过程中应该避免接触皮肤、眼睛和呼吸道，应该在通风良好的环境下操作，并使用个人防护装备如实验手套和护目镜。另外，福林酚应远离火源和高温环境，储存在阴凉、干燥的地方。
最后更新：2024-04-09 19:43:38
酚试剂 - 介绍
Folin-Ciocalteu（福林-西奥卡特）试剂即Folin-酚试剂中的试剂Ⅱ,也就是福林酚B液 Folin-酚试剂中的试剂Ⅰ是A液。福林酚A液配置方法：1) 4%碳酸钠溶液2) 0.2mol/L氢氧化钠溶液3) 1%硫酸铜溶液4) 2%酒石酸钾钠溶液。临用前将（1）与（2）等体积配制碳酸钠—氢氧化钠溶液。（3）与（4）等体积配制成硫酸铜—酒石酸钾钠溶液。然后这两种试剂按50:1的比例配合，即成Folin—酚试剂A。此试剂临用前配制，一天内有效。
最后更新：2022-10-16 17:31:01
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