Molecular Formula | C41H44N2O14S4 |
Molar Mass | 917.05306 |
Storage Condition | -20℃ |
Physical and Chemical Properties | Bioactive Cy5.5 (Sulfo-Cyanine5.5) is a near infrared fluorescent dye (Ex = 673 nm, Em = 707 nm) used to label biomolecules, such as peptides, proteins and oligonucleotides. |
Reference Show more | 1. [IF=18.952] Zheng Di-Wei et al."An orally delivered microbial cocktail for the removal of nitrogenous metabolic waste in animal models of kidney failure."Nat Biomed Eng. 2020 Jul;4(9):853-862 |
background
Cy5.5 is a kind of cyano dye. The definition of cyano is "synthetic dye, its general formula is R2N[CH = CH]nCH = N R2, and it is decomposed into R2N = CH[CH = CH]nNR2 (N is a decimal), where nitrogen and partially conjugated chains usually form part of a heterocyclic system, such as imidazole, pyridine, pyrirole, quinoline and thiazole." Cyano dyes are used in industrial biotechnology (labeling, analysis, biomedical imaging).
Because cyanic acid can produce brighter and more stable fluorescence, it can well replace traditional dyes such as fluorescein and rhodamine. Cy3 and Cy5 are the most commonly used, usually used for the detection of two colors. Cy3 emits green-yellow fluorescence (about 550 nm excitation, about 570 nm emission), while Cy5 emits fluorescence in the red region (about 650 nm excitation, about 670 nm emission).
Cy5 has become a popular substitute for red fluorescent dyes due to its high extinction coefficient (1 nanomol can be found with naked eyes in gel electrophoresis). Cy5.5 is a near infrared (IR) fluorescent emission dye (excitation/emission maximum 678/694 nm).
fluorescent dye
fluorescent dyes can produce fluorescent dyes. It can mainly absorb visible light waves and ultraviolet light waves, and can convert ultraviolet light into visible light waves with longer wavelengths and reflect them. For example, acidic eosin, fluorescent yellow, mercury, and some disperse dyes. Most of them are compounds containing benzene rings or heterocyclic rings with conjugated double bonds.
fluorescent dyes have bright colors. besides printing and dyeing fiber fabrics, they can also be used as some special signs (such as dark symbols) and military tracking. Fluorescent dyes contain groups that can be conjugated with nucleic acids, carbohydrates and peptides. The conjugates are generally stable and have better solvent discoloration effects than other fluorescent substances or color markers under ultraviolet and visible light irradiation.
in vitro studies
Cy5.5 contains a free amine group which can be conjugated with a variety of functionalities, including NHS esters, and epoxides. The excitation and emission maximum are 678 and 694 nm, respectively. Cy5.5 has been applied in various fluorescent based experiments. Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. Cy5.5 is cost-effective and its labeling chemistries are easy to perform, making it suitable for potential anti-cancer drug development.
In vivo studies
Cy5.5-labeled factor VIIa is developed for imagining cancer. Cy5.5 labeled with these targeting proteins specifically localize to the tumor xenografts for at least 14 days but unconjugated Cy5.5 does not localize to any xenografts or organs. This method of imaging anti-tissue factor in the tumor VECs may be useful in detecting primary tumors and metastases as well as monitoring in vivo therapeutic responses. pH/temperature sensitive magnetic nanogels conjugated with Cy5.5-labled lactoferrin (Cy5.5-Lf-MPNA nanogels) are developed as a promising contrast agent for preoperative MRI and intraoperative fluorescence imaging of glioma.