CS-2186

CS-2186 - Names and Identifiers

Name	5-(phenylmethyl)-N-[(3S)-2,3,4,5-tetrahydro-5-methyl-4-oxo-1,5-benzoxazepin-3-yl]-3-isoxazolecarboxamide
Synonyms	GSK481 CS-2186 GSK481 - GSK'481 5-(phenylmethyl)-N-[(3S)-2,3,4,5-tetrahydro-5-methyl-4-oxo-1,5-benzoxazepin-3-yl]-3-isoxazolecarboxamide 3-Isoxazolecarboxamide, 5-(phenylmethyl)-N-[(3S)-2,3,4,5-tetrahydro-5-methyl-4-oxo-1,5-benzoxazepin-3-yl]-
CAS	1622849-58-4

CS-2186 - Physico-chemical Properties

Molecular Formula	C21H19N3O4
Molar Mass	377.39
Density	1.35±0.1 g/cm3(Predicted)
Boling Point	677.0±55.0 °C(Predicted)
Solubility	DMSO: 30 mg/mL mother liquor preservation: sub-package and freeze storage to avoid repeated freezing and thawing;-20 ℃,1 month;-80 ℃,6 months (after dilution, the solution temperature is low and storage may precipitate, try to use it now) Cell experiment: Dissolve with DMSO first: dilute with culture medium then, and the dilution process is recommended to be carried out in stages to avoid too fast concentration change leading to compound precipitation. If the compound is precipitated during the dilution process, it can be redissolved by ultrasound. During dilution, ensure that the final concentration of DMSO in the working fluid should be below 0.1% as far as possible, and the maximum should not exceed 0.5%, and set up a DMSO control group with corresponding concentration. Animal experiment: Dissolve with DMSO first: dilute with water or normal saline, etc. The dilution process is recommended to be carried out in sections to avoid excessive concentration changes leading to compound pr
pKa	11.68±0.20(Predicted)
Storage Condition	-20℃
Use	GSK481 is a Highly Potent and Monoselective Receptor Interacting Protein 1 Kinase Inhibitor (RIP1 inhibitor). The recent discovery of the role of receptor interacting protein 1 (RIP1) kinase in tumor necrosis factor (TNF)-mediated inflammation has led to its emergence as a highly promising target for the treatment of multiple inflammatory diseases.
Target	RIP1
In vitro study	GSK481 can inhibit the phosphorylation of human and mouse RIP1(WT)S166, which is overexpressed in HEK293T cells. The inhibitory effect of GSK481 on human and cynomolgus RIP1 is almost the same, but the efficacy of RIP on non-primate is much worse.

CS-2186 - Reference

Reference

1: Harris PA, King BW, Bandyopadhyay D, Berger SB, Campobasso N, Capriotti CA,Cox JA, Dare L, Dong X, Finger JN, Grady LC, Hoffman SJ, Jeong JU, Kang J,Kasparcova V, Lakdawala AS, Lehr R, McNulty DE, Nagilla R, Ouellette MT, Pao CS,Rendina AR, Schaeffer MC, Summerfield JD, Swift BA, Totoritis RD, Ward P, ZhangA, Zhang D, Marquis RW, Bertin J, Gough PJ. DNA-Encoded Library ScreeningIdentifies Benzo[b][1,4]oxazepin-4-ones as Highly Potent and MonoselectiveReceptor Interacting Protein 1 Kinase Inhibitors. J Med Chem. 2016 Mar10;59(5):2163-78. doi: 10.1021/acs.jmedchem.5b01898. PubMed PMID: 26854747.

CS-2186 - Preparation solution concentration reference

	1mg	5mg	10mg
1 mM	2.65 ml	13.249 ml	26.498 ml
5 mM	0.53 ml	2.65 ml	5.3 ml
10 mM	0.265 ml	1.325 ml	2.65 ml
5 mM	0.053 ml	0.265 ml	0.53 ml

Last Update：2024-01-02 23:10:35

CS-2186 - Reference Information

biological activity	GSK481 is an inhibitor of RIP1(Receptor Interacting Protein Kinase1, RIPK1), which inhibits RIP1 to hinder cell necrosis.
target	TargetValue RIP1
Target	Value
in vitro study	GSK481 can inhibit the phosphorylation of human and mouse RIP1(WT)S166 overexpressed in HEK293T cells. GSK481 has almost the same inhibitory effect on human and cynomolgus monkey RIP1, but it is much less effective on non-primate RIP.

Last Update：2024-04-09 21:54:55