Molecular Formula | C20H23BCl2N2O9 |
Molar Mass | 517.122 |
Storage Condition | − 20 ℃, closed, dry |
Use | Overview Nattokinase natto produces a serine protease from Bacillus Subtilis natto during fermentation. Nattokinase has fibrinolytic activity and can treat and prevent thrombotic diseases. |
Abstract:
The Nattokinase gene was amplified from Bacillus subtilis genomic DNA secreting Nattokinase by PCR, and its nucleotide sequence was determined. The expression vector of Nattokinase gene was constructed by Gene Recombination technology and expressed in E. Coli. SDS-polyacrylamide gel electrophoresis showed that the expressed protein accounted for 15.2% of the thalli protein, and the expression product had thrombolytic activity by agarose-fibrin plate method.
Key words:
Nattokinase gene sequence expression clone thrombolytic agents
DOI:
CNKI:SUN:SWHZ.0.1999-06-013
cited:
year:
1999
Xiong Xiaohui , Liang Jianguang bear strong
Abstract:
The liquid fermentation production of Bacillus subtilis (BSN) was studied. The effects of fermentation conditions (temperature, inoculum size, initial pH, liquid volume) and medium composition (carbon source, nitrogen source, metal ions, etc.) on the yield of Nattokinase were investigated; on the basis of optimizing the fermentation conditions and culture medium, the amplification experiment of 5L fermentor was carried out, and the yield of enzyme was 2 25 IU/mL.
Key words:
Bacillus natto Nattokinase liquid fermentation
DOI:
10.3321/j.issn:0253-990X.2004.01.016
cited:
year:
2004
Wang Junju , Li peifan , Off Red
Abstract:
objective to study the activity and anticoagulant effect of Nattokinase (NK). Methods the activity of NK was determined by fibrin plate method. The whole blood clotting time, plasma re-Calcium Time, thrombin time, rat thromboplastin time, thrombin-induced fibrin coagulation time; The prothrombin time of rats was determined by Quick method. Results the activity of solid powder NK was 5 6 5 5 10U/g, which was 3.13 times of that of urokinase, and the activity of liquid NK was 15 2 34U/ml;NK can significantly prolong the rabbit whole blood coagulation time, plasma re Calcium Time, thrombin time, rat thromboplastin time, thrombin induced fibrin clotting time, rat prothrombin time. Conclusion NK has influence on the three stages of coagulation process.
Key words:
Nattokinase activity assay anticoagulant effect
degree year:
2004
DOI:
10.3969/j.issn.1005-1678.2004.05.007
cited:
Liu Yufeng , Wang Jinying , Sun, shore , Wang Zhanbin , Yang Hongbo
DOI:
CNKI:SUN:SWJS.0.2000-02-017
cited:
year:
2000