mPEG-Fluoresein,MPEG-FITC - Names and Identifiers
mPEG-Fluoresein,MPEG-FITC - Physico-chemical Properties
mPEG-Fluoresein,MPEG-FITC - Introduction
mPEG-Fluoresein,MPEG-FITC(mPEG-Fluorescein,MPEG-FITC) is a fluorescently labeled compound commonly used in biomedical and biochemical research. Its main properties and uses are as follows:
Nature:
1. Chemical structure: Methoxy polyethylene glycol and fluorescein (Fluorescein) are linked by chemical bonds.
2. fluorescence properties: mPEG-FITC can emit green fluorescence at the appropriate excitation wavelength.
Use:
1. Biomarkers: mPEG-FITC can be combined with biological macromolecules (such as proteins, antibodies, etc.) to achieve visual positioning and research of target molecules in biological samples.
2. Cell tracing: mPEG-FITC can be taken up by cells for applications such as cell tracking, cell proliferation, and cell detection.
3. Protein structure study: mPEG-FITC can be used as a fluorescent probe for protein quantitative analysis and protein structure study.
Preparation Method:
mPEG-FITC can be prepared by coupling fluorescein with methoxy polyethylene glycol. The specific method is generally a condensation reaction between fluorescein and methoxypolyethylene glycol containing active hydroxyl groups under alkaline conditions to form a coupling product. After the reaction, the final product is obtained by solvent extraction, purification and the like.
Safety Information:
mPEG-FITC are generally less toxic, but still need to be handled and used properly. During the operation, it is necessary to follow the laboratory's safe operation specifications to avoid direct contact with skin, eyes and mucous membranes. In case of accidental contact, rinse immediately with plenty of water. In addition, it should be stored in a closed container, away from high temperature and direct sunlight.
Last Update:2024-04-09 20:52:54