Maleimidocaproyl-monomethylauristatin E MedChemExpress (MCE)

Product Name	Mc-MMAE
Synonyms	McMMAE Mc-MMAE Mc MMAE Maleimidocaproyl-monomethylauristatin E Maleimidocaproyl-monomethylauristatin E fandachem L-Valinamide, N-[6-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)-1-oxohexyl]-N-methyl-L-valyl-N-[(1S,2R)-4-[(2S)-2-[(1R,2R)-3-[[(1R,2S)-2-hydroxy-1-methyl-2-phenylethyl]amino]-1-methoxy-2-methyl-3-oxopropyl]-1-pyrrolidinyl]-2-methoxy-1-[(1S)-1-methylpropyl]-4-oxobutyl]-N-methyl-
CAS	863971-24-8
EINECS
Chemical Formula	C49H78N6O10
Molecular Weight	911.18
inchi
Package	1 mg;5 mg;10 mg;25 mg;50 mg
Price	Email to quote
Descriptions	Mc-MMAE Mc-MMAE MedChemExpress (MCE) HY-15741 863971-24-8 Maleimidocaproyl-monomethylauristatin E 99.93% 4°C, stored under nitrogen The compound is unstable in solutions, freshly prepared is recommended. Room temperature in continental US Descriptions Mc-MMAE Mc-MMAE MedChemExpress (MCE) HY-15741 863971-24-8 Maleimidocaproyl-monomethylauristatin E 99.93% 4°C, stored under nitrogen The compound is unstable in solutions, freshly prepared is recommended. Room temperature in continental US may vary elsewhere. Mc-MMAE is a protective group (maleimidocaproyl)-conjugated monomethyl auristatin E (MMAE), which is a potent tubulin inhibitor. Mc-MMAE is a agent-linker conjugate for ADC. Synthesis of maleimidocaproyl-MMAE (mc-MMAE) requires the addition of maleimidocaproic acid to a solution of MMAE in dichloromethane followed by the addition of diethyl cyanophosphonate and diisopropylethylamine[1]. Horseradish peroxidase (HRP) is thiolated with 2-iminothiolane and conjugated to mc-MMAE to generate the HRP-MMAE reporter enzyme-drug conjugate. Briefly, a thiolation reaction mixture containing 0.2 mM HRP (8 mg/mL) and 50 mM 2-iminothiolane in 25 mM sodium borate decahydrate (Na2B4O7•10H2O) buffer (pH 9.0) is incubated for 1 hour at 37°C. Unreacted 2-iminothiolane is removed by passage through a PD-10 desalting column equilibrated in PBS (pH 7.4). Peak fractions are pooled and mc-MMAE is coupled to thiolated HRP (HRP-SH) at a molar ratio of 3:1. The final conjugation reaction mixture contained 80 μM HRP-SH (3.2 mg/mL) in sodium borate buffer [50 mM H3BO3, 50 mM NaCl (pH 8.0) 80% v/v] and 240 μM mc-MMAE in ice-cold CH3CN (20% v/v). After 30 minutes on ice, the reaction is terminated with a 20-fold molar excess of free cysteine (4.8 mM) before PD-10 chromatography. Peak fractions containing HRP-MMAE (exchanged into PBS) are pooled and evaluated for extent of modification using the thiol-reactive dye, Alexa Fluor 594 C5 maleimide[1]. Auristatin \| \| \| \| \| \| \| \| \| \| [1]. Sanderson RJ, et al. In vivo drug-linker stability of an anti-CD30 dipeptide-linked auristatin immunoconjugate. Clin Cancer Res. 2005 Jan 15 11(2 Pt 1):843-52. [Content Brief]
Last Update	2025-04-01 14:45:47

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