MOPS MedChemExpress (MCE)

Product Name	3-Morpholinopropanesulfonic Acid
Synonyms	MOPS TIMTEC-BB SBB009133 LABOTEST-BB LT01595956 LABOTEST-BB LT00455082 MOPS, CONCENTRATE SOLUTION MOPS, Free Acid, ULTROL Grade MORPHOLINOPROPANE SULFONIC ACID Synonyms MOPS TIMTEC-BB SBB009133 LABOTEST-BB LT01595956 LABOTEST-BB LT00455082 MOPS, CONCENTRATE SOLUTION MOPS, Free Acid, ULTROL Grade MORPHOLINOPROPANE SULFONIC ACID 3-MORPHOLINOPROPANESULFONIC ACID 4-MORPHOLINEPROPANESULFONIC ACID 3-MorpholinopropanesuIfonic Acid 3-Morpholinopropanesulfonic Acid 3-N-morpholinopropansulfonic acid 3-MORPHOLINOPROPANESULPHONIC ACID MOPS, Free Acid, MB Grade (1.01545) 4-(MORPHOLINOPROPANE SULFONIC ACID) 3-(4-Morpholino)propanesulphonic acid 3-(4-Morpholino)propane sulfonic acid 3-(morpholin-4-yl)propane-1-sulfonic acid MOPS 3-(N-Morpholino)propanesulfonic acid MOPS, 10X Liquid Concentrate,Molecular Biology Grade MOPS, Molecular Biology Grade 3-(N-Morpholino)propanesulfonic acid, Molecular Biology Grade
CAS	1132-61-2
EINECS	214-478-5
Chemical Formula	C7H15NO4S
Molecular Weight	209.26
inchi	InChI=1/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
Package	10 mM * 1 mL;500 mg
Price	Email to quote
Descriptions	MOPS MOPS MedChemExpress (MCE) HY-D0859 1132-61-2 98.0% RT, protect from light In solvent -80°C 2 years -20°C 1 year Room temperature in continental US Descriptions MOPS MOPS MedChemExpress (MCE) HY-D0859 1132-61-2 98.0% RT, protect from light In solvent -80°C 2 years -20°C 1 year Room temperature in continental US may vary elsewhere. MOPS are commonly used as buffers in biology. MOPS buffer maintains the pH of mammalian cell culture media. MOPS and Tris buffer also have inhibitory effects on TfCut2 and LCC hydrolases, inhibiting the rate of hydrolyzing PET films. MOPS may also interfere with calcium binding, translocation, and utilization in vascular smooth muscle cells. Preparation of Keratinocyte and Microbial Culture Medium Using MOPS[3] Materials: MOPS: 164 mM RPMI-1640 medium L-glutamine: 2 mM Sodium bicarbonate: 2.0 g/L Sterile phosphate-buffered saline (PBS, pH 7.2) Normal oral keratinocytes (NOK) or human keratinocyte cell line (HaCat) Microorganisms: Candida albicans SC5314, Staphylococcus aureus ATCC25923 Sterile water Steps: 1. Preparation of MOPS Buffer: Dissolve 164 mM of MOPS in 1 L of sterile water, and adjust the pH to 7.0. Add 2 mM of L-glutamine and 2.0 g/L of sodium bicarbonate, stirring until fully dissolved. Filter the solution using a sterile filtration device to ensure sterility. 2. Preparation of Keratinocyte Culture Medium: Mix the prepared MOPS buffer with RPMI-1640 medium at a 1:1 ratio to obtain MOPS-containing medium. Seed NOK or HaCat cells into a 24-well plate, adding approximately 4.5 × 104 cells per well with an appropriate amount of RPMI/MOPS medium. Incubate the cells in a 37°C, 5% CO2 incubator until confluent (approximately 48 hours). 3. Preparation of Microbial Culture Medium: Prepare the Candida albicans and Staphylococcus aureus strains, culturing them in suitable media until they reach the logarithmic growth phase. Resuspend the microorganisms in MOPS medium at a concentration of approximately 107 cells/mL. Inoculate the microorganisms into a 24-well plate, adding an appropriate amount of MOPS medium, and incubate at 37°C in a shaker at 75 rpm for 90 minutes. 4. Co-culture of Keratinocytes and Microorganisms: Co-culture keratinocytes and microorganisms in a 24-well plate, adding MOPS medium to maintain the pH around 7.0. The co-culture duration can be set based on experimental needs, with a recommendation to limit the time to under 12 hours to reduce the cytotoxicity of MOPS. 5. Cell Viability Assay: Use the MTT assay to measure cell viability, sampling every 2 hours, adding MTT/PBS solution, incubating at 37°C for 4 hours, and recording the results. Notes: MOPS is relatively safe for short-term experiments, but it may significantly reduce cell viability after 4 hours, so it is recommended to limit the experiment duration to 4-12 hours. \| \| \| \| \| \| \| \| \| \| [1]. Steven D Carson, et al. MOPS and coxsackievirus B3 stability. Virology. 2017 Jan 15 501:183-187. [Content Brief] [2]. Juliane Schmidt, et al. Effect of Tris, MOPS, and phosphate buffers on the hydrolysis of polyethylene terephthalate films by polyester hydrolases. FEBS Open Bio. 2016 Jul 20 6(9):919-27. [Content Brief] [3]. Dias Kde C, et al. Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth. J Microbiol Methods. 2016 Jun 125:40-2. [Content Brief]
Last Update	2025-04-01 14:45:47

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