Serratia marcescens nuclease MedChemExpress (MCE)

Product Name	MUNG BEAN NUCLEASE
Synonyms	R;P1;S1;EC;3.1.30.1;3.1.30.2;NUCLEASE;BENZONASE;ENDONUCLEASE;BENZONASE(R);MUNG BEAN NUCLEASE
CAS	9025-65-4
EINECS
Chemical Formula
Molecular Weight
inchi
Package	5 KU;10 KU;25 KU
Price	Email to quote
Descriptions	Nuclease, Serratia marcescens Nuclease, Serratia marcescens MedChemExpress (MCE) HY-131160 9025-65-4 Serratia marcescens nuclease 95.00% Please store the product under the recommended conditions in the Certificate of Analysis. Room temperature in continental US Descriptions Nuclease, Serratia marcescens Nuclease, Serratia marcescens MedChemExpress (MCE) HY-131160 9025-65-4 Serratia marcescens nuclease 95.00% Please store the product under the recommended conditions in the Certificate of Analysis. Room temperature in continental US may vary elsewhere. Serratia marcescens nuclease (EC 3.1.30.2) is a nonspecific nuclease. Serratia marcescens nuclease has broad utility due to its potent digestive activity toward both DNA and RNA. Product Information An endonuclease from Serratia marcescens that can completely digest all forms of DNA and RNA (including single-stranded, double-stranded, linear and circular) into 5'monophosphate oligonucleotides of 3-5 bases in length. It has no protein activity and is highly specific. It is expressed in a yeast system and is suitable for the removal of nucleic acids in various proteins and biological products. The reaction conditions are mild, the reaction time is short, and the nucleic acid removal efficiency is high. The storage solution is: 20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerol Instructions1. Sample preparation 1) Adherent cells: remove the culture medium, wash the cells with PBS, and remove the supernatant. 2) Suspended cells: collect the cells by centrifugation, wash the cells with PBS, centrifuge at 6,000 rpm for 10 min, and collect the precipitate. 3) Escherichia coli: collect the bacteria by centrifugation, wash once with PBS, centrifuge at 8,000 rpm for 5 min, and collect the precipitate. 2. Sample processing The collected cell pellets are lysed at a ratio of mass (g) to volume (mL) of 1:10~20. Cells can also be lysed mechanically or chemically on ice or at room temperature (1 g of cells is approximately 109). 3. Enzyme addition 1) Add an appropriate amount of MgCl2 to adjust the Mg2+ concentration in the reaction system to within the range of 1-5 mM, and adjust the pH to 8-9. 2) Add enzymes at a ratio of 250 U to digest 1 g of cell pellets, and incubate at 37°C for more than 30 minutes. You can also select the addition scheme according to the recommended usage in the table above, increase the enzyme amount within a certain range, and the digestion time will be reduced accordingly. Note: Enzyme activity is affected by factors such as ion concentration, reaction temperature and pH. It is recommended to explore the optimal concentration for the first use. 4. Supernatant acquisition Centrifuge at 12,000 rpm for 30 minutes to obtain the cell lysate supernatant, and then conduct subsequent related experiments. Note: If the solution is a high-salt solution, slightly acidic or alkaline, and contains a higher concentration of detergent or denaturant, the amount of enzyme should be appropriately increased or the incubation time should be extended. \| \| \| \| \| \| \| \| \| \| [1]. "Vafina G, et al. Endonuclease from Gram-Negative Bacteria Serratia marcescens Is as Effective as Pulmozyme in the Hydrolysis of DNA in Sputum. Front Pharmacol. 2018 9:114. Published 2018 Feb 16. doi:" [Content Brief] [2]. "Friedhoff P, et al. A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. Protein Expr Purif. 1994 5(1):37-43. " [Content Brief]
Last Update	2025-04-01 14:45:47

MedChemExpress (MCE) also provides

Thalidomide-4-O-C2-NH2

Category: Signaling Pathways
CAS: 2341840-99-9
Last Update: 2025-04-01 14:45:47

PPARα-MO-1

Category: Signaling Pathways
CAS: 810677-36-2
Last Update: 2025-04-01 14:45:47

Netupitant N-oxide

Category: Signaling Pathways
CAS: 910808-11-6
Last Update: 2025-04-01 14:45:47

MK-0812

Category: Signaling Pathways
CAS: 624733-88-6
Last Update: 2025-04-01 14:45:47

TS-011

Category: Signaling Pathways
CAS: 339071-18-0
Last Update: 2025-04-01 14:45:47

SCH 530348 sulfate

Category: Signaling Pathways
CAS: 705260-08-8
Last Update: 2025-04-01 14:45:47

BAG 956

Category: Signaling Pathways
CAS: 853910-02-8
Last Update: 2025-04-01 14:45:47

YM758

Category: Signaling Pathways
CAS: 312752-85-5
Last Update: 2025-04-01 14:45:47


Supplier Name	MedChemExpress (MCE)
Contact	sales
Tel	609-228-6898
Mobile	609-228-6898
QQ
Email	sales@medchemexpress.com; tech@medchemexpress.com
Website	https://www.medchemexpress.com/
Wechat

Request for quotation

Supplier Contact