H100 MedChemExpress (MCE)

Product Name	H100
Synonyms	H100;643727-55-3
CAS	643727-55-3
EINECS
Chemical Formula	C18H16N2O6S
Molecular Weight	388.39
inchi
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Descriptions	H100 H100 MedChemExpress (MCE) HY-100322 643727-55-3 Please store the product under the recommended conditions in the Certificate of Analysis. Room temperature in continental US Descriptions H100 H100 MedChemExpress (MCE) HY-100322 643727-55-3 Please store the product under the recommended conditions in the Certificate of Analysis. Room temperature in continental US may vary elsewhere. H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter and the Band 3 anion exchanger, but no effect against KCl cotransporter, in human erythrocytes. H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter (NKCC) and the Band 3 anion exchanger (AE), but no effect against KCl cotransporter (KCC), in human erythrocytes. H100 (0.1 mM) shows 63% and 74% inhibition of NKCC and AE in human erythrocytes[1]. 35SO42- efflux is used as a convenient measure of the activity of the Band 3 anion exchanger (AE). Erythrocytes are suspended into MBS containing Na2SO4 rather than NaCl and incubated at 37°C for 30 min. The cell suspension is then centrifuged (3,000 g, 5 min) and the supernatant removed. The above procedure is repeated twice to ensure the intracellular replacement of Cl- with SO42-. To load the erythrocytes with radiolabel, the packed cells are re-suspended to approximately 50% haematocrit in a solution containing 1 part SO42- MBS and 9 parts of a medium containing (in mM) 300 sucrose and 10 MOPS (pH 7.4, 300 ± 5 mOsm) and placed in a microcentrifuge tube. 10 μCi of 35SO42- is then added and the suspension incubated at 37°C for 1 h. At the end of this period, the cells are then washed four times by centrifugation (10,000 g, 10 s) in ice-cold SO42- MBS. All inhibitors (H100) are dissolved in either DMSO or MBS and are added to the cell suspensions prior to the addition of the radioisotope[1]. Cl- transport[1] In Vitro H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter (NKCC) and the Band 3 anion exchanger (AE), but no effect against KCl cotransporter (KCC), in human erythrocytes. H100 (0.1 mM) shows 63% and 74% inhibition of NKCC and AE in human erythrocytes[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> H100 Related Antibodies \| \| \| \| \| \| \| \| \| \| [1]. Culliford S1, et al. Specificity of classical and putative Cl(-) transport inhibitors on membrane transport pathways in human erythrocytes. Cell Physiol Biochem. 2003 13(4):181-8. [Content Brief]
Supplier Website	https://www.medchemexpress.com/H100.html
Last Update	2025-05-21 16:50:25

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