9001-37-0
葡萄糖氧化酶(glucose oxidase from aspergillus niger)
CAS: 9001-37-0
化学式:
性质
淡黄色或灰黄色粉末，溶于水成黄绿色溶液，不溶于醚、氯仿、丁醇、吡啶、甘油、乙二醇、二噁烷、1，3一丙二醇和甲酰胺。能被50%丙酮或66%甲醇所沉淀。来自霉菌的葡萄糖氧化酶的分子量
约150000，等电点pH 4.2～4.3，最适pH 5.5～5.8，最适温度30～35℃，酸、碱和高温能使其破坏。
用途
常用诊断用酶。葡萄糖的分析。除去过量的葡萄糖或氧，是维生素C及维生素B12的制剂的稳定剂。制尿糖和血糖试纸。蛋白质脱糖、罐头酒类的贮藏、制药工业等。
安全性
5g包装，密封干燥，4℃以下保存，溶液应防止冻结。
| 中文名 | 葡萄糖氧化酶 |
| 英文名 | glucose oxidase from aspergillus niger |
| 别名 | 葡糖氧化酶 氧化葡萄酶 葡萄糖氧化酶 葡萄糖氧化酶 来源于黑曲霉 GLUCOSE OXIDASE 来源于黑曲霉 |
| 英文别名 | notatin 1.1.3.4 oxidaseglucose glucose oxidase from aspergillus niger |
| CAS | 9001-37-0 |
| EINECS | 232-601-0 |
| 密度 | 1.00g/mLat 20°C |
| 蒸汽压 | 0.004Pa at 25℃ |
| 溶解度 | 易溶于水，完全不溶于乙醚、氯仿、丁醇、吡啶、甘油、乙二醇等有机溶剂，50％丙酮、66％的甲醇能使其沉淀。 |
| PH值 | 4.5 |
| 存储条件 | -20°C |
| 稳定性 | 稳定。与强氧化剂不相容。防潮。 |
| 外观 | 溶液 (清除) |
| 颜色 | yellow |
| Merck | 14,4460 |
| 物化性质 | 近白色粉末至棕色液体。溶于水，几不溶于乙醇，氯仿和乙醚。 |
| MDL号 | MFCD00131182 |
| 危险品标志 | Xn - 有害物品 Xi - 刺激性物品  |
| 风险术语 | R42 - 吸入可能致敏。 R36/37/38 - 刺激眼睛、呼吸系统和皮肤。 |
| 安全术语 | S45 - 若发生事故或感不适，立即就医(可能的话，出示其标签)。 S22 - 切勿吸入粉尘。 S23 - 切勿吸入蒸汽。 S36 - 穿戴适当的防护服。 S26 - 不慎与眼睛接触后，请立即用大量清水冲洗并征求医生意见。 S24/25 - 避免与皮肤和眼睛接触。 |
| WGK Germany | 1 |
| RTECS | RQ8452000 |
| FLUKA BRAND F CODES | 3-10 |
| 海关编号 | 35079090 |
| 上游原料 | 高岭土 |
| 下游产品 | 葡萄糖酸内酯 |
| 参考资料 展开查看 | 1. 张志国 王硕 生庆海.HPLC-ELSD法检测液态奶中的低聚半乳糖[J].食品与机械 2011 27(03):68-70+75. 2. 周冉 宋玉品 刘晓妹 等. 仿生硅化固定化葡萄糖氧化酶与辣根过氧化酶研究[J]. 食品工业科技 2017 38(002):221-225. 3. 向宇 鲁群 谭军 张久亮 刘睿 朱和平.响应面分析优化枸杞浑浊汁饮料稳定工艺研究[J].核农学报 2017 31(11):2154-2163. 4. 王誉涵, 林家亮, 徐宏. 基于一种新型二茂铁修饰方法的葡萄糖氧化酶电极研究[J]. 分析试验室, 2018, 37(12):1380-1383. 5. 熊俐, 曹新志, 吕开斌,等. 复合菌和酶制剂在麸皮面包品质改良上的应用[J]. 食品研究与开发, 2013, 000(016):22-25. 6. 常波, 叶丹, 王敏,等. 磁性碳纳米管修饰POD-GOD双酶系统葡萄糖传感器的研究[J]. 成都师范学院学报, 2018, 034(009):96-103. 7. 王菲菲, 高山, 牟德华. 脱糖方式对山楂中特征成分含量的影响[J]. 食品工业, 2020(3):170-175. 8. 沈王庆, 陈蓉, 赵秀蓉,等. 葡萄糖氧化酶和柠檬汁对甘蔗汁保鲜效果的研究[J]. 天然产物研究与开发, 2016(28):1533. 9. 孔亚访, 樊爱萍. 金纳米簇增强的化学发光反应及在葡萄糖检测中的应用[J]. 分析科学学报, 2018. 10. 王新坤, 王月明, 刘超,等. 青稞碳水化合物构成与血糖稳定品质的关系[J]. 中国粮油学报, 2019(8):34-41. 11. 王新坤, 刘超, 杨清梅,等. 青稞籽粒β-葡聚糖含量及不同加工方式对其变化的影响[J]. 山东农业科学, 2019, 051(002):124-130. 12. 孔晓雪, 李蕴涵, 李柚,等. 葡萄糖氧化酶和谷氨酰胺转氨酶对发酵麦麸面团加工品质的影响[J]. 食品工业科技, 2019, 40(9). 13. 田双起,张锦霞,胡浩杰,王新伟,王彦波.远红外制备甘薯生全粉对面团流变学特性影响[J].食品工业,2020,41(09):55-59. 14. Qu, Fei, et al. 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"Gluten development and water distribution in bread dough influenced by bran components and glucose oxidase." LWT 137 (2021): 110427.https://doi.org/10.1016/j.lwt.2020.110427 19. Qi, Guohua, et al. "Glucose oxidase probe as a surface-enhanced Raman scattering sensor for glucose." Analytical and bioanalytical chemistry 408.26 (2016): 7513-7520. 20. Liu, Wei, et al. "Fluorometric and colorimetric sensor array for discrimination of glucose using enzymatic-triggered dual-signal system consisting of Au@ Ag nanoparticles and carbon nanodots." Sensors and Actuators B: Chemical 265 (2018): 310-317.https://d 21. Liu, Tingting, et al. "Facile strategy to prepare a metalloporphyrin-based hydrophilic porous organic polymer with enhanced peroxidase-like activity and high stability for colorimetric detection of H2O2 and glucose." Colloids and Surfaces B: Biointerfaces 22. Xie, Siqi, et al. 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"Clinically colorimetric diagnostics of blood glucose levels based on vanadium oxide quantum dots enzyme mimics." Microchemical Journal 153 (2020): 104352.https://doi.org/10.1016/j.microc.2019.104352 27. Wang, Yan, et al. "Hemin-porous gC 3 N 4 hybrid nanosheets as an efficient peroxidase mimic for colorimetric and visual determination of glucose." Microchimica Acta 186.7 (2019): 1-10. 28. Dan Li, Yanxiao Jiang, Sihan Chen, Qingnan Zhao, Yue Zhang, Wei Wang, Ying Sun, Pinyi Ma, Daqian Song, Xinghua Wang,A simple and sensitive assay of alkaline phosphatase activity in serum by fluorescent silicon nanoparticles based on inner filter effect,S 29. Zhang, Fangmei, et al. "Dopamine-modified Mn-doped ZnS quantum dots fluorescence probe for the sensitive detection of tyrosinase in serum samples and living cells imaging." Sensors and Actuators B: Chemical 256 (2018): 1069-1077.https://doi.org/10.1016/j.s 30. 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[IF=7.46] Wei Liu et al."Fluorometric and colorimetric sensor array for discrimination of glucose using enzymatic-triggered dual-signal system consisting of Au@Ag nanoparticles and carbon nanodots."Sensor Actuat B-Chem. 2018 Jul;265:310 35. [IF=7.1] Fangmei Zhang et al."Dopamine-modified Mn-doped ZnS quantum dots fluorescence probe for the sensitive detection of tyrosinase in serum samples and living cells imaging."Sensor Actuat B-Chem. 2018 Mar;256:1069 36. [IF=6.057] Dalong Yu et al."Iodophenol blue-enhanced luminol chemiluminescence and its application to hydrogen peroxide and glucose detection."Talanta. 2016 Jan;146:655 37. [IF=6.057] Meng-Meng Liu et al."A colorimetric assay for sensitive detection of hydrogen peroxide and glucose in microfluidic paper-based analytical devices integrated with starch-iodide-gelatin system."Talanta. 2019 Aug;200:511 38. [IF=5.833] Zhang Fangmei et al."Enzymatic determination of uric acid using water-soluble CuInS/ZnS quantum dots as a fluorescent probe."Microchim Acta. 2018 Nov;185(11):1-8 39. [IF=5.339] Fangmei Zhang et al."Rapid aqueous synthesis of CuInS/ZnS quantum dots as sensor probe for alkaline phosphatase detection and targeted imaging in cancer cells."Talanta. 2018 Nov;189:411 40. [IF=5.339] Dongqin Yang et al."A nanocluster-based fluorescent sensor for sensitive hemoglobin detection."Talanta. 2017 Aug;170:233 41. [IF=5.268] Tingting Liu et al."Facile strategy to prepare a metalloporphyrin-based hydrophilic porous organic polymer with enhanced peroxidase-like activity and high stability for colorimetric detection of H2O2 and glucose."Colloid Surface B. 2019 Jun;178:137 42. [IF=4.952] Meng Niu et al."Comparative study on protein polymerization in whole-wheat dough modified by transglutaminase and glucose oxidase."Lwt Food Sci Technol. 2018 Apr;90:323 43. [IF=4.464] Fang Yuan et al."Split photoelectrochemistry for the immunoassay of α-fetoprotein based on graphitic carbon nitride."J Electroanal Chem. 2016 Dec;783:226 44. [IF=4.142] Qi Guohua et al."Glucose oxidase probe as a surface-enhanced Raman scattering sensor for glucose."Anal Bioanal Chem. 2016 Oct;408(26):7513-7520 45. [IF=4.142] Wang Xiaoyan et al."A novel switchable fluorescent sensor for facile and highly sensitive detection of alkaline phosphatase activity in a water environment with gold/silver nanoclusters."Anal Bioanal Chem. 2019 Feb;411(5):1009-1017 46. [IF=4.098] Dongmei Yao et al."A facile SERS strategy for quantitative analysis of trace glucose coupling glucose oxidase and nanosilver catalytic oxidation of tetramethylbenzidine."Spectrochim Acta A. 2019 Jun;216:146 47. [IF=3.361] Yao-hui Wu et al."The screening of metal ion inhibitors for glucose oxidase based on the peroxidase-like activity of nano-Fe3O4."Rsc Adv. 2017 Oct;7(75):47309-47315 48. [IF=16.806] Jiangang Liu et al."A Biomimetic Plasmonic Nanoreactor for Reliable Metabolite Detection."Adv Sci. 2020 May;7(10):1903730 49. [IF=10.618] Yu Cui et al."A ratiometric fluorescence platform based on boric-acid-functional Eu-MOF for sensitive detection of H2O2 and glucose."Biosens Bioelectron. 2019 Jun;135:208 50. [IF=10.618] Tao Chen et al."A molecularly imprinted nanoreactor with spatially confined effect fabricated with nano-caged cascaded enzymatic system for specific detection of monosaccharides."Biosens Bioelectron. 2021 Sep;188:113355 51. [IF=9.229] Xiqing Cheng et al."Biomimetic Metal–Organic Framework Composite-Mediated Cascade Catalysis for Synergistic Bacteria Killing."Acs Appl Mater Inter. 2020;12(33):36996–37005 52. [IF=8.897] Cheng Xiqing et al."One-step synthesis of thermally stable artificial multienzyme cascade system for efficient enzymatic electrochemical detection."Nano Res. 2019 Dec;12(12):3031-3036 53. [IF=8.198] Xiqing Cheng et al."Metal–Organic Framework as a Compartmentalized Integrated Nanozyme Reactor to Enable High-Performance Cascade Reactions for Glucose Detection."Acs Sustain Chem Eng. 2020;8(48):17783–17790 54. [IF=7.46] Tao Chen et al."Specific detection of monosaccharide by dual-channel sensing platform based on dual catalytic system constructed by bio-enzyme and bionic enzyme using molecular imprinting polymers."Sensor Actuat B-Chem. 2020 Oct;320:128430 55. [IF=7.46] Yangyang Zhao et al."Photothermal-enhanced tandem enzyme-like activity of Ag2-xCuxS nanoparticles for one-step colorimetric glucose detection in unprocessed human urine."Sensor Actuat B-Chem. 2020 Feb;305:127420 56. [IF=7.46] Dan Li et al."A simple and sensitive assay of alkaline phosphatase activity in serum by fluorescent silicon nanoparticles based on inner filter effect."Sensor Actuat B-Chem. 2020 Mar;307:127589 57. [IF=7.46] Qian Su et al."Dual-emissive fluorescence and phosphorescence detection of cholesterol and glucose based on carbon dots-cyanuric acid complex quenched by MnO2 nanosheets."Sensor Actuat B-Chem. 2021 May;335:129715 58. [IF=7.393] Siqi Xie et al."AIE-active metal–organic frameworks: facile preparation, tunable light emission, ultrasensitive sensing of copper(II) and visual fluorescence detection of glucose."J Mater Chem C. 2020 Aug;8(30):10408-10415 59. [IF=6.558] Tianran Lin et al."Multicolor and photothermal dual-mode assay of alkaline phosphatase based on the UV light-assisted etching of gold nanorods."Anal Chim Acta. 2021 Oct;1181:338926 60. [IF=5.833] Yang Fencheng et al."Fe/C magnetic nanocubes with enhanced peroxidase mimetic activity for colorimetric determination of hydrogen peroxide and glucose."Microchim Acta. 2019 Jul;186(7):1-8 61. [IF=5.833] Wang Yan et al."Hemin-porous g-C3N4 hybrid nanosheets as an efficient peroxidase mimic for colorimetric and visual determination of glucose."Microchim Acta. 2019 Jul;186(7):1-10 62. [IF=5.467] Wenning Yang et al."Bioinspired hierarchical CoAl-LDH/MFe2O4(Ni, Zn, Co) as peroxidase mimics for colorimetric detection of glucose."Appl Clay Sci. 2019 Nov;181:105238 63. [IF=5.316] Wenning Yang et al."Biomass-derived hierarchically porous CoFe-LDH/CeO2hybrid with peroxidase-like activity for colorimetric sensing of H2O2 and glucose."J Alloy Compd. 2020 Jan;815:152276 64. [IF=5.268] Wenning Yang et al."A colorimetric strategy for ascorbic acid sensing based on the peroxidase-like activity of core-shell Fe3O4/CoFe-LDH hybrid."Colloid Surface B. 2020 Apr;188:110742 65. [IF=4.952] Fan Xiao et al."Gluten development and water distribution in bread dough influenced by bran components and glucose oxidase."Lwt Food Sci Technol. 2021 Feb;137:110427 66. [IF=4.821] Xucan Yuan et al."Fluorescence and scattering based dual-optical signals ratiometric sensing and logic gate device for acetylcholinesterase activity assay."Microchem J. 2021 Nov;170:106768 67. [IF=4.821] Yan Niu et al."A “turn-on” fluorescent probe for ultra-sensitive detection of β-galactosidase."Microchem J. 2021 Jul;166:106205 68. [IF=4.821] Weishuai Ma et al."Clinically colorimetric diagnostics of blood glucose levels based on vanadium oxide quantum dots enzyme mimics."Microchem J. 2020 Mar;153:104352 69. [IF=4.616] Meng-Meng Liu et al."Improving quantitative control and homogeneous distribution of samples on paper-based analytical devices via drop-on-demand inkjet printing."Analyst. 2019 Jun;144(13):4013-4023 70. [IF=4.616] Guoning Chen et al."Facile one-step targeted immobilization of an enzyme based on silane emulsion self-assembled molecularly imprinted polymers for visual sensors."Analyst. 2019 Dec;145(1):268-276 71. 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9001-37-0 - 性质
可信数据
淡黄色或灰黄色粉末，溶于水成黄绿色溶液，不溶于醚、氯仿、丁醇、吡啶、甘油、乙二醇、二噁烷、1，3一丙二醇和甲酰胺。能被50%丙酮或66%甲醇所沉淀。来自霉菌的葡萄糖氧化酶的分子量
约150000，等电点pH 4.2～4.3，最适pH 5.5～5.8，最适温度30～35℃，酸、碱和高温能使其破坏。
最后更新：2024-01-02 23:10:35
9001-37-0 - 用途
可信数据
常用诊断用酶。葡萄糖的分析。除去过量的葡萄糖或氧，是维生素C及维生素B12的制剂的稳定剂。制尿糖和血糖试纸。蛋白质脱糖、罐头酒类的贮藏、制药工业等。
最后更新：2022-01-01 11:16:47
9001-37-0 - 安全性
可信数据
5g包装，密封干燥，4℃以下保存，溶液应防止冻结。
最后更新：2022-01-01 11:16:47
9001-37-0 - 活力定义
One unite will oxidize one micromole of glucose per min at pH5.7 at 37℃
最后更新：2023-08-16 21:32:38
9001-37-0 - Molecular weight
approx 150 kDa (SDS-PAGE).
最后更新：2023-08-16 21:32:38
9001-37-0 - Isoelectric point
4.2.
最后更新：2023-08-16 21:32:38
9001-37-0 - 简介
葡萄糖氧化酶（glucose oxidase）是一种重要的酶类物质，主要存在于两种微生物中——黄曲霉和阿洛热性放线菌中。
葡萄糖氧化酶的主要作用是催化葡萄糖的氧化反应，将葡萄糖转化为葡萄糖酸，并释放出氧气。
葡萄糖氧化酶的制法一般通过培养产生该酶的微生物来获得。从黄曲霉或阿洛热性放线菌中分离出产生葡萄糖氧化酶的菌株，并进行纯化和固化。将纯化的酶制成粉末或液体的形式以便使用。
就安全性而言，葡萄糖氧化酶是一种相对安全的酶类物质。像所有的酶一样，如果酶被误食或与眼睛接触，可能会引起不适。在使用葡萄糖氧化酶时应该采取适当的个人防护措施，并遵守相关化学品的处理和储存规范。
葡萄糖氧化酶的主要作用是催化葡萄糖的氧化反应，将葡萄糖转化为葡萄糖酸，并释放出氧气。
葡萄糖氧化酶的制法一般通过培养产生该酶的微生物来获得。从黄曲霉或阿洛热性放线菌中分离出产生葡萄糖氧化酶的菌株，并进行纯化和固化。将纯化的酶制成粉末或液体的形式以便使用。
就安全性而言，葡萄糖氧化酶是一种相对安全的酶类物质。像所有的酶一样，如果酶被误食或与眼睛接触，可能会引起不适。在使用葡萄糖氧化酶时应该采取适当的个人防护措施，并遵守相关化学品的处理和储存规范。
最后更新：2024-04-09 21:01:54
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产品名: 葡萄糖氧化酶 来源于黑曲霉 去供应商网站查看 询盘CAS: 9001-37-0
产地: 上海
规格: G914877
价格: 50KU-526,50KU-492,10KU-188,250KU-2299,1g-936,5g-2560,100mg-172,500mg-498,25g-5999,200KU-1890
库存: 现货
电话: 13816333479/17821173903
手机: 13816333479/17821173903
电子邮件: wangrui@macklin.cn
QQ: 2885703204/288570320
微信: 19863730987/17821173903
现货供应
产品名: 葡萄糖氧化酶 来源于黑曲霉 去供应商网站查看 询盘CAS: 9001-37-0
产地: 上海
规格: G914877
价格: 50KU-526,50KU-492,10KU-188,250KU-2299,1g-936,5g-2560,100mg-172,500mg-498,25g-5999,200KU-1890
库存: 现货
电话: 13816333479/17821173903
手机: 13816333479/17821173903
电子邮件: wangrui@macklin.cn
QQ: 2885703204/288570320
微信: 19863730987/17821173903
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